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fluorescence lifetime imaging microscopy flim  (Lambert Instruments BV)


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    Structured Review

    Lambert Instruments BV fluorescence lifetime imaging microscopy flim
    Fluorescence Lifetime Imaging Microscopy Flim, supplied by Lambert Instruments BV, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescence+lifetime+imaging+microscopy+flim/LIFA+FLIM/arxiv__2502__13003-103-9-15
    Average 93 stars, based on 116 article reviews
    fluorescence lifetime imaging microscopy flim - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: [2502.13003] Fluorescent molecular rotor-based polymer materials for local microviscosity mapping in microfluidic channels
    Article Snippet: .. Lifetimes τF were measured using a LIFA system for Fluorescence Lifetime Imaging Microscopy (FLIM) from Lambert Instruments, equipped with a LED source of wavelength 451nm, close to the maximum of absorption of the FMR. ..

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Imaging:

    Article Title: [2502.13003] Fluorescent molecular rotor-based polymer materials for local microviscosity mapping in microfluidic channels
    Article Snippet: .. Lifetimes τF were measured using a LIFA system for Fluorescence Lifetime Imaging Microscopy (FLIM) from Lambert Instruments, equipped with a LED source of wavelength 451nm, close to the maximum of absorption of the FMR. ..

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Microscopy:

    Article Title: [2502.13003] Fluorescent molecular rotor-based polymer materials for local microviscosity mapping in microfluidic channels
    Article Snippet: .. Lifetimes τF were measured using a LIFA system for Fluorescence Lifetime Imaging Microscopy (FLIM) from Lambert Instruments, equipped with a LED source of wavelength 451nm, close to the maximum of absorption of the FMR. ..

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Inverted Microscopy:

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).

    Article Title: Polarized Cell Motility Induces Hydrogen Peroxide to Inhibit Cofilin via Cysteine Oxidation
    Article Snippet: Following a PBS wash and the addition of SF and phenol red free medium, DCFDA fluorescence was measured on a Tecan Safire II microplate reader (excitation wavelength = 485 nm; emission wavelength = 528 nm; bandwidth = 5 nm). .. All fluorescence lifetime imaging microscopy (FLIM) was carried out on a Nikon TE 2000 inverted microscope fitted with a Lambert Instruments FLIM Attachment (LIFA) and a Yokogawa CSU22 spinning disk unit. .. Images were acquired with the 488 nm laser and a Plan Apo 100x/1.40 oil objective (Nikon).



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    Phasor plots: ( A ) the <t>fluorescence</t> <t>lifetime</t> <t>imaging</t> microscopy phasor plot of rhodamine 6G 5 μM and ( B ) sample No 1 with rhodamine 6G 5 μM as a reference.
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    Image Search Results


    Phasor plots: ( A ) the fluorescence lifetime imaging microscopy phasor plot of rhodamine 6G 5 μM and ( B ) sample No 1 with rhodamine 6G 5 μM as a reference.

    Journal: International Journal of Nanomedicine

    Article Title: Ultra-pure, water-dispersed Au nanoparticles produced by femtosecond laser ablation and fragmentation

    doi: 10.2147/IJN.S44163

    Figure Lengend Snippet: Phasor plots: ( A ) the fluorescence lifetime imaging microscopy phasor plot of rhodamine 6G 5 μM and ( B ) sample No 1 with rhodamine 6G 5 μM as a reference.

    Article Snippet: The Nikon Eclipse Ti microscope with a Lambert fluorescence lifetime imaging microscopy (FLIM) attachment was used to carry out experiments on the quenching of the rhodamine 6G (Rh6G) dye lifetime in the presence of gold nanoparticles.

    Techniques: Fluorescence, Imaging, Microscopy

    Determination of the fluorescence lifetime, τ = A/(Bω), from a phasor plot, where ω is the modulation frequency; see text for details.

    Journal: International Journal of Nanomedicine

    Article Title: Ultra-pure, water-dispersed Au nanoparticles produced by femtosecond laser ablation and fragmentation

    doi: 10.2147/IJN.S44163

    Figure Lengend Snippet: Determination of the fluorescence lifetime, τ = A/(Bω), from a phasor plot, where ω is the modulation frequency; see text for details.

    Article Snippet: The Nikon Eclipse Ti microscope with a Lambert fluorescence lifetime imaging microscopy (FLIM) attachment was used to carry out experiments on the quenching of the rhodamine 6G (Rh6G) dye lifetime in the presence of gold nanoparticles.

    Techniques: Fluorescence

    Schematic of Pt–Au bimetallic system that catalyzes the decomposition of hydrogen peroxide yielding ionic species which generate an electric field (red streamlines) that drives fluid flow (black streamlines). L = 10 mm is the length of bielectrode, and H is the height of the cell, where H = 1500 μm for the FLIM setup and H = 500 μm for the fluid flow setup.

    Journal: The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

    Article Title: Electrocatalytic Reaction Driven Flow: Role of pH in Flow Reversal

    doi: 10.1021/acs.jpcc.1c06458

    Figure Lengend Snippet: Schematic of Pt–Au bimetallic system that catalyzes the decomposition of hydrogen peroxide yielding ionic species which generate an electric field (red streamlines) that drives fluid flow (black streamlines). L = 10 mm is the length of bielectrode, and H is the height of the cell, where H = 1500 μm for the FLIM setup and H = 500 μm for the fluid flow setup.

    Article Snippet: The proton concentrations close to the surface of the bielectrode were determined with fluorescence lifetime imaging microscopy (FLIM; LIFA, Lambert Instruments, The Netherlands).

    Techniques:

    Lifetime vs proton concentration and calibration curve for FLIM measurements on semilog scale. Data was plotted within the pH range 5–6, where the fluorescence dye is sensitive to protonation. Solid line corresponds to the best fit to a Boltzmann sigmoid model, and the dashed lines refer to the 95% confidence interval.

    Journal: The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

    Article Title: Electrocatalytic Reaction Driven Flow: Role of pH in Flow Reversal

    doi: 10.1021/acs.jpcc.1c06458

    Figure Lengend Snippet: Lifetime vs proton concentration and calibration curve for FLIM measurements on semilog scale. Data was plotted within the pH range 5–6, where the fluorescence dye is sensitive to protonation. Solid line corresponds to the best fit to a Boltzmann sigmoid model, and the dashed lines refer to the 95% confidence interval.

    Article Snippet: The proton concentrations close to the surface of the bielectrode were determined with fluorescence lifetime imaging microscopy (FLIM; LIFA, Lambert Instruments, The Netherlands).

    Techniques: Concentration Assay, Fluorescence

    Proton concentration profile along the junction of the bielectrode for simulation (bulk pH 5.62) at channel heights and FLIM results. The solid lines represent the simulation results, and the open symbols depict the FLIM data. The distance was nondimensionalized by the length of the bielectrode, L = 10 000 μm. The proton concentration was averaged at the electrode for FLIM.

    Journal: The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

    Article Title: Electrocatalytic Reaction Driven Flow: Role of pH in Flow Reversal

    doi: 10.1021/acs.jpcc.1c06458

    Figure Lengend Snippet: Proton concentration profile along the junction of the bielectrode for simulation (bulk pH 5.62) at channel heights and FLIM results. The solid lines represent the simulation results, and the open symbols depict the FLIM data. The distance was nondimensionalized by the length of the bielectrode, L = 10 000 μm. The proton concentration was averaged at the electrode for FLIM.

    Article Snippet: The proton concentrations close to the surface of the bielectrode were determined with fluorescence lifetime imaging microscopy (FLIM; LIFA, Lambert Instruments, The Netherlands).

    Techniques: Concentration Assay